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博士后学术沙龙(第86期)
文:医学院 图:医学院 来源:医学院 时间:2023-06-23 1641

       为搭建我校博士后之间的学术交流平台,促进学术水平提升,学校人力资源部博士后和教师引进管理办公室组织开展博士后学术沙龙活动。本次沙龙由我校博士后袁伦、吴炳火、张静超和卢永凤分享其研究成果,诚挚邀请感兴趣的师生参加。

  一、时 间:2023年6月28日(周三)09:30

  二、地 点:沙河校区医学院楼213会议室

                         腾讯会议ID:973-413-299

  三、主办单位:人力资源部博士后和教师引进管理办公室

  四、承办单位:医学院   电子科技大学博士后联谊会

  五、活动安排:

  报告一:   

  (1)主题:乳液模板法制备功能化明胶细胞支架及其性能研究

  (2)主讲人:袁伦  医学院博士后

  (3)交流内容:

  本报告的主要内容是乳液模板法制备微观结构可控明胶细胞支架及其功能化应用。在研究过程中建立乳化参数与支架微观孔道结构的对应关系,研发孔道结构可控细胞支架制备方法,探索细胞支架功能化的各种方法,以满足实际应用的各种不同需求。本报告主要从以下三个部分展开:(1) 利用乳液模板法制备明胶细胞支架,探究不同的明胶支架对小鼠成纤维细胞(L929)和小鼠胚胎成骨细胞前体细胞(MC3T3-E1)细胞生长状况的影响,探索不同类型细胞所需最佳生长孔径;(2) 采用乳液模板法制备具有皮肤再生所需最优微观孔道结构的明胶支架,并通过不同策略对其进行功能化改性,制备具有抗菌活性的细胞支架,并评估其用于促进伤口快速愈合的可行性;(3) 针对不同微环境中骨再生的需求,制备可注射自愈合型水凝胶或异构双层屏障膜,并评估其修复效果。

  (4)主讲人简介:

  袁伦,电子科技大学医学院博士后,在四川大学获得生物工程学士学位,随后分别在四川大学李德富教授和穆畅道教授的指导下开展生物化工硕士和博士研究,并于2022年获得博士学位。主要研究方向包括乳液模板法制备孔道结构可控细胞支架,可注射自愈合型水凝胶的制备以及功能化生物材料用于骨缺损修复。

  报告二:   

  (1)主 题:能量代谢调控造血干细胞命运决定

  (2)主讲人:吴炳火  医学院博士后

  (3)交流内容:

  主讲人长期从事骨髓造血干细胞(Hematopoietic stem cells,HSCs)及其微环境研究,并重点关注能量代谢调控HSCs命运决定的机制,利用多种策略从分子、细胞到动物等不同层面研究HSCs的功能调控机制。本次汇报主要内容总结如下:

  ①HSCs处于骨髓低氧微环境中,以低氧糖酵解为主的细胞代谢状态维持了HSCs的干性、增殖和分化能力。细胞氧化磷酸化的增加和低氧代谢状态的失衡是HSCs功能损伤和衰老的关键因素之一。报告人研究发现,抑制鞘氨醇激酶SphK2,能够增强HSCs对低氧的反应性、保护HSCs的低氧糖酵解状态,通过这种全新的代谢干预策略,能够促进HSCs的功能维持和抗衰老作用。

  ②HSCs的长期造血能力维持需要保持其不进入细胞周期的静息态,进入细胞周期后活化的HSCs长期造血能力丢失。静息态HSCs维持低葡萄糖摄取和以糖酵解为主的低能量代谢状态,并维持较低的蛋白质合成水平,这是其长期功能维持的关键。进入活化态的HSCs,由于葡萄糖摄取和能量代谢的增加导致细胞活性氧的积累,同时蛋白质合成的增加会导蛋白质稳态失衡及异常折叠蛋白的堆积,从而损伤其长期造血能力,诱发蛋白质稳态失衡相关的细胞死亡。报告人在前期研究中揭示了HSCs中能量代谢和蛋白质稳态维持之间的关联及分子机制,并发现代谢物烟酰胺核糖能够调控HSCs的氨基酸分解代谢、促进蛋白质稳态维持,保护其长期造血能力,对于促进HSCs的体外扩增和增强移植后HSCs的造血重建能力具有重要临床意义。

  (4)主讲人简介:

  吴炳火,医学博士;2016年本科毕业于四川大学华西医学中心基础医学(基地班)专业,2022年博士毕业于中山大学中山医学院干细胞与组织工程教育部重点实验室。主要围绕骨髓微环境开展研究工作,研究方向包括造血干细胞代谢调控与命运决定、间充质干细胞与骨发育及造血支持等,研究成果以(共同)第一作者发表于Cell Stem Cell、Blood等期刊。

  报告三:     

  (1)主 题:Oscillation of type IV pili regulated by the circadian clock in cyanobacterium Synechococcus elongatus PCC7942

  (2)主讲人: 张静超  医学院博士后 

  (3)交流内容:

  Type IV pili (TFP) are closely related to cell motilities and natural transformation. However, for photosynthetic cyanobacteria, the molecular and ecological functions of their TFP have rarely been reported. Here, by labeling pili in cyanobacterium Synechococcus elongatus PCC 7942 (Syn7942), we have quantitatively characterized the TFP and its-driven twitching motility in situ in Syn7942 at the single-cell level for the first time. Importantly, we discovered an oscillating pattern of TFP in accordance with the light and dark periods during light-dark cycles, which positively correlates to the oscillating pattern of the natural transformation efficiency. We further showed that the internal circadian clock plays an important role in regulating the oscillating pattern of TFP, which is supported at the molecular level by the qRT-PCR measurements of 16 TFP-related genes. This study adds a detailed picture toward to the gap between TFP and its relations to circadian regulations in Syn7942.

  (4)主讲人简介:

  Jingchao Zhang received the Bachelor degree from Agricultural University of Hebei and Master and Ph.D. degree from the Tianjin University, respectively. She currently works as a Postdoctoral Fellow in the University of Electronic Science and Technology and Sichuan Provincial People's Hospital. Dr. Zhang mainly engaged in cross-cutting research in the field of soft matter and biomedical engineering. her main research interests include imaging, detection and behavior characterization of bacteria and their interactions with host cells at the single-cell level.

   报告四:     

  (1)主 题:Research on Precision Diagnosis Technology of Lung Cancer Based on Distance Dependence Plasmon Resonance Coupling

  (2)主讲人: 卢永凤  医学院博士后 

  (3)交流内容:

  In this talk, we briefly discuss the precise diagnostic techniques for lung cancer with the metal surface plasmon resonance, which can effectively improve the optical performance of extracellular vesicles and free proteins.

  There are three issues: (1) the antibody-specific recognition biomarkers, (2) Metal nanoparticles linked to different antibodies, (3) After metal nanoparticles are connected to different antibodies, different biomarkers are recognized, and the signal output is differentiated.

  First, we surveyed the literature and selected specific biomarkers in the pleural effusion of lung cancer: CEA. Then, according to the method in the literature, we use metal nanoparticles to connect biomarkers to form a better sandwich detection structure. Finally, by detecting the sensitivity and specificity of the sandwich detection structure, the enhancement of its signal makes the signal of the nanoparticle and the biomarker present a one-to-one correspondence, which sensitivity is higher than that of ELISA.

  (4)主讲人简介:

  Dr. Lu received the Ph.D. degree from the Fuzhou University in 2022. She currently works as a Postdoctoral Fellow in the School of Medicine of UESTC. Dr. Lu is working in the field of biomarker detection in cancer micro environment with metal nanomaterials.

    

                                              人力资源部博士后和教师引进管理办公室

                                                                  2023年6月21日

 

 

 


编辑:李果  / 审核:李果  / 发布:陈伟

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